Optical microscopy

Fluorescence microscopy can provide rich insights into organic and inorganic samples in life science and materials sciences. A wide range of laser wavelengths is required to match the fluorophore of interest. Confocal laser scanning microscopes (LSM) are commonly used, which can reconstruct three-dimensional images with high optical resolution and contrast.

TOPTICA's offer

TOPTICAs diode lasers and multi-laser engines offer high beam quality, high power stability and excellentlifetime at a very competetive price. In addition, our lasers and laser engines feature optimized modulation capabilities for superior performance. Microscopy-optimized laser sources reduce investment costs and optical system complexity.

For non-linear microscopy applications TOPTICA offers a broad wide range of femtosecond fiber lasers. The FemtoFiber laser family has proven its outstanding performance and suitability for in-vivo imaging and other advanced non-linear microscopy techniques.

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Non-linear microscopy techniques, such as 2-photon fluorescence microscopy or second harmonic generation (SHG) microscopy, have become key technologies in biological imaging, enabling three-dimensional, non-invasive studies of tissue with subcellular resolution on the micrometer scale. In contrast to conventional linear fluorescence microscopy, the nonlinear character and the longer excitation wavelengths offer the key advantages of greater  probing depth and lower phototoxicity.

TOPTICA's offer

TOPTICA’s femtosecond fiber lasers are easy to use and offer optimal performance. In particular, TOPTICA’s FemtoFiber ultra lasers are  ideally suited for imaging green (GFP), red (RFP), blue (BFP) fluorescent proteins, autofluorescence, or SHG signals to address highly relevant biological questions. It is no wonder that these laser systems are already widely used in nonlinear microscopes worldwide.

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Non-linear fluorescence microscopy is the state-of-the-art technique for direct spatiotemporal visualization of neurons and neuronal activity in living animals. The key fluorescent proteins for such experiments are based on green fluorescent protein (GFP) and red fluorescent protein (RFP), which can be excited by 2-photon absorption at 920 nm and 1050 nm, respectively.

In recent years, there has been a rapid shift toward fixed-wavelength mode-locked femtosecond fiber lasers. These laser systems offer a cost-effectiveand easy-to-use alternative for two-photon microscopy applications, especially in neuroscience.

TOPTICA's offer

TOPTICA’s femtosecond fiber lasers offer optimal performance and ease of use. Especially for neuroscience applications, TOPTICA’sFemtoFiber ultra lasers are ideally suited to address biological questions by imaging green (GFP) and red fluorescent proteins (RFP), and are already widely used in nonlinear microscopes.

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