FemtoFiber ultra dual-color

Optically synchronized laser system for multi-color non-linear microscopy

  • Two synchronized laser lines for multi-color non-linear imaging
  • Shared oscillator design for all-optical synchronization
  • Fixed delay between two colors with minimum jitter
  • Single electronic trigger output as reference for TCSPC and gated detection

In two-photon microscopy, simultaneous multi-color imaging provides rich insight into biological samples . Especially, metabolic imaging greatly benefits from simultaneous NADH and FAD measurements. With the FemtoFiber ultra dual-color two FemtoFiber ultra are optically synchronized and allow simultaneous imaging with two different laser colors. No laser wavelength tuning is required. A shared oscillator design guarantees all-optical synchronization and hence a fixed, defined delay between the two laser colors with minimum jitter. In addition, only a single electronic trigger output serves as reference for TCSPC electronics in Fluorescence lifetime imaging (FLIM) as well as gated detectors.

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  • Specification

    Center wavelength 920 nm and 780 nm 920 nm and 1050 nm
    Average output power > 1 W at 780 nm, > 1.5 W at 920 nm > 1.5 W at 920 nm, > 5 W at 1050 nm
    Pulse duration <150 fs W at 780 nm, < 100 fs at 920 nm < 100 fs at 920 nm, < 100 fs at 1050 nm
    Repetition rate 80 MHz
    Motorized dispersion precompensation (GDD) -30,000 fs² ... 0 fs² at 780 nm, -40000 fs² ... +1000 fs² at 920, independent per wavelength -40000 fs² ... +1000 fs², independent per wavelength
    Integrated power control (AOM, optional) > 1 MHz AOM modulation bandwidth, independent per wavelength, analogue and digital modulation available
    Dimensions supply unit Twice 131 x 484 x 600 mm³ (19 inch rack format, 3U)
    Dimensions laser head Twice 77 x 165 x 300 mm³ (H x W x D)
    Further information FemtoFiber ultra 780, FemtoFiber ultra 920 FemtoFiber ultra 920, FemtoFiber ultra 1050

     

  • Applications
  • Literature

    Becker Wolfgang, Axel Bergmann, Alexander Jelzow, Antje Neubauer, Angelika Rück, Konrad Birkmeier, and Patrick Leisching. “Metabolic Imaging by Simultaneous 2-Photon FLIM of NAD(P)H and FAD.” In Multiphoton Microscopy in the Biomedical Sciences XX, 11244:112440L. International Society for Optics and Photonics, 2020.